Topo pcr ii dual promotor thermofisher
WebTOPO TA Cloning Dual Promoter kits are for fast, efficient cloning and subsequent in vitro transcription. These kits include the pCRII-TOPO TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious rest WebTOPO® TA Cloning® Dual Promoter kits are for fast, efficient cloning and subsequent in vitro transcription. These kits include the pCR™II-TOPO® TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO® cloning is the most reliable cloning method, featuring a 3-step protocol and 5 ...
Topo pcr ii dual promotor thermofisher
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WebFast—5-minute, room temperature reaction; Simple—add restriction sites and/or universal primer sites to either end of your PCR product in just 3 easy steps; Efficient—up to 95% of … WebThese kits include the pCR™II-TOPO™ TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO™ cloning is the …
WebPcrii Topo Dual Promoter Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Average 86 stars, based on 1 Price from $9.99 to $1999.99 WebDual Promoter Pcrii Topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article …
WebLinearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products. Similar to pCR®2.1-TOPO® but with an SP6 promoter. ... Thermo Fisher …
Web2 days ago · Locations of dual-sgRNA target sites were shown in red arrow, primer pair F/R (Mlo2478/2479) were used for PCR testing deletion in flail1 and flail2. B. Selection of red fluorescent seed from T1 plants after transformation of the pKIR1.1-based T-DNA construct to induce the FLAIL2 deletion. Arrows represented selected seeds.
WebThermo Fisher pcrii topo dual promoter cloning vector Pcrii Topo Dual Promoter Cloning Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, … country songs about hellWebProduce PCR product Amplify DNA encoding a eukaryotic promoter of choice using Taq polymerase and your own protocol. End the PCR reaction with a final 7 to 10 minute extension step. Perform the TOPO® ® Cloning Reaction 1. Set up one of the following TOPO Cloning reactions using the reagents in the order shown. brewery near pigeon forgeWeby effective method to generate recombinant baculoviruses by combining the ease of blunt-end TOPO® cloning with the efficiency of site-specific transposition technology of the Bac-to-Bac® System. The Bac-to-Bac® TOPO® Expression System is available separately with a choice of pFastBac™/CT-TOPO® or pFastBac™/NT- TOPO® donor plasmids, which are … brewery near ravenna ohioWebTOPO TA Cloning Dual Promoter kits are for fast, efficient cloning and subsequent in vitro transcription. These kits include the pCRII-TOPO TA vector with dual T7 and SP6 … country songs about jailWebTOPO™-TA: Content And Storage: Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: country songs about leaving townWebInvitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10F' Chemically Competent E. coli Designed for cloning PCR products directly from a PCR reaction in just 5 minutes 754.00 EUR - … brewery near panama city beachWebAs far as I know, there are at least five different kits for cloning blunt-end PCR product. 1. NEB PCR Cloning Kit. 2. Thermo fisher CloneJET PCR Cloning Kit 3. Thermo fisher Zero Blunt... country songs about hating someone