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Expressing Transgenes That Exceed the Packaging Capacity
Webb20 apr. 2024 · A linear range for rAAV5 size and titer determination with MADLS was established to be 4.4 × 10 11 -8.7 × 10 13 cp/mL for the nominally full rAAV5 samples and 3.4 × 10 11 -7 × 10 13 cp/mL for the nominally empty rAAV5 samples with 3-8% and 10-37% CV for the full and empty rAAV5 samples, respectively. Webbno longer solely comprised of monomer AAVs. Addition-ally, the true size distribution of the aggregate peak is obscured since the majority of the particles are co- eluting in the void … bailando tarantela
Analytical ultracentrifugation (SV-AUC / SE-AUC) - Coriolis Pharma
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