Phospho stat3 flow cytometry

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August undefined, 2024

WebGeneral phospho-protein staining technique for flow cytometry. (Step 1) A heterogeneous sample of cells is treated with two different stimuli, A and B (i.e., cytokines, growth factors, drugs,... WebApr 15, 2024 · a STAT3 activation in un-transfected and transfected with a cDNA coding for murine IL-11R murine myoblasts (C2C12) without cytokine (-) and after stimulation with HIL-11 (200 ng/ml), IL-11 (200 ng ...

Pacific Blue™ Mouse anti-Stat3 (pY705) - BD Biosciences

WebBrand: BD Phosflow™ Alternative Name: Signal Transducer and Activator of Transcription-1; STAT91; ISGF-3; CANDF7 Reactivity: Human (QC Testing), Mouse (Tested in Development) Isotype: Mouse IgG2a Immunogen: Phosphorylated Human Stat1 Peptide Application: Intracellular staining (flow cytometry) (Routinely Tested) Vol. Per Test: 20 µl RRID: … WebPhosphoflow cytometry is increasingly being used as a tool for the discovery of biomarkers used in the treatment and monitoring of disease and therapy. The ability to measure numerous phospho-protein targets simultaneously at a single cell level accurately and rapidly provides significant advantages … raw chocolate recipe uk

stat3 Primary Antibodies - Thermo Fisher

WebOct 3, 2024 · Flow cytometry analysis was performed on BD FACSCanto II cytometer. Data analysis was performed with FCS Express 6 software. The target cells were gated on FSC/SSC plot to remove debris, followed by a singlet gate on FSC-H/FSCW plot. ... SKBr3 cancer cell lines probed for total and phospho-STAT3 by Western blotting. (B) … WebA specific band was detected for Phospho-STAT3 (Y705) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Intracellular Staining by Flow Cytometry View Larger Detection of Phospho-STAT3 (Y705) in IFN alpha-treated Daudi Human Cell Line by Flow Cytometry. simple club anomalie des wassers

Phospho-Stat3 (Tyr705) (D3A7) XP - Cell Signaling …

Anti-STAT3 (phospho S727) antibody (ab30647) Abcam

WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-STAT3 (phospho S727) antibody [E121-31] (ab32143) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment – … WebOct 17, 2024 · The surface expression of mIL-6R and gp130 on CD4+ T cells from 50 μl of fresh whole blood was analyzed by flow cytometry on the same day as pSTAT3 detection by phosflow cytometric analysis; this was assessed in a proportion of patients in each group (3-week group, n = 5; 4-week group, n = 7; 5-week group, n = 5; control group, n = 8 ... raw chrysocollaWebFlow cytometric analysis of Stat3 (pY705). Human whole blood was collected in the presence of heparin. Whole blood was either left unstimulated (unshaded) or stimulated … raw chocolate suppliers

"WebNov 15, 2013 · In order to validate a phospho-specific flow cytometry assay assessing phospho-signal transducer and activator of transcription factor 5 (p-STAT5) as a new diagnostic tool for JMML, we examined 22 samples from children with JMML and 47 controls. CD33+/CD34+ cells from 22 patients with JMML showed hyperphosphorylation … " - Phospho stat3 flow cytometry

Phospho stat3 flow cytometry

Human Phospho-STAT3 (S727) Antibody MAB4934: R&D Systems

WebWestern blot analysis for Stat3 (pY705). A431 cells (Human epithelial carcinoma; ATCC CRL-1555) were either left untreated (lane 1) or treated with 100 ng/ml EGF for 5 minutes at 37°C (lane 2). The top panel was probed with a mouse anti-Stat3 antibody measuring for total Stat3 (Cat. No. 610189) while the bottom panel was probed with a 1:500 ... WebProtocol for Phospho-Flow Cytometry Preparation (Provided by Donald J McGuire and Dr. Chander Raman) Phospho Flow Methanol perm Cell stimulation Do your regular cell …

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WebJan 1, 2012 · Flow cytometry can be used to study STAT phosphorylation on a per cell basis. Cells are fixed, permeablized, and stained with antibodies that specifically bind to … WebFeb 19, 2024 · Costunolide is a naturally occurring sesquiterpene lactone that demonstrates various therapeutic actions such as anti-oxidative, anti-inflammatory, and anti-cancer properties. Costunolide has recently emerged as a potential anti-cancer agent in various types of cancer, including colon, lung, and breast cancer. However, its mode of action in …

WebFlow Cytometry CHARACTERIZATION This antibody is tested by direct flow cytometric analysis of human cells. WORKING DILUTIONS ... (green), using Phospho-Stat3 (Tyr705) (D3A7) XP Alexa Fluor 488 conjugate compared to nonspecific control antibody (red), and flow cytometric analysis of Jurkat cells, untreated (blue) or IFN-α treated (green), using ... WebFlow cytometry quantification of phospho-STAT3, phospho-STAT5 and expression of Bcl-2/Bcl-XL after TQ treatment. Phosphorylation of STAT3 (A) and STAT5 (B) as well as the …

WebHuman Phospho-STAT3 (S727) Antibody Summary Species Reactivity Human Specificity Detects human STAT3 when phosphorylated at S727. Source Monoclonal Mouse IgG 2B Clone # 788335 Purification Protein A or G purified from hybridoma culture supernatant Immunogen Phosphopeptide containing the human STAT3 S727 site Formulation WebApr 12, 2024 · The effect of FLU on STAT3 expression and activation in A172 and T98G cells. (A) The time dependence of FLU effect on STAT3 and phospho-STAT3 (Tyr705) expression. Cell were treated with 0.5 µM ...

WebMOLT4 cells are characterized by higher basal STAT3 phosphorylation. These data document that phospho-specific flow cytometry reveals a high variability in intrinsic as well as modulated signaling networks across different T-ALL cell lines.

WebDec 1, 2024 · In summary, STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates the EMT–MET switch within the distinct molecular subtypes of CSCs to complete the metastatic process. simple club aktionspotentialWebSep 1, 2016 · The following phospho-specific monoclonal antibodies were used in 3 different panels during the flow cytometry protocol: Alexa Fluor ®647 conjugated anti-Stat4 … simple clown makeup halloweenWebMar 1, 2024 · The role of STAT3 in B cell responses to IFNα was studied using pharmacological inhibitors and PBMCs from STAT3-deficient individuals. Results: … simpleclub angebotWebDec 1, 2024 · In summary, STAT3 phosphorylation at tyrosine 705 and serine 727 differentially regulates the EMT–MET switch within the distinct molecular subtypes of CSCs to complete the metastatic process. simpleclub app windows downloadWebApr 10, 2024 · The cell phenotype was observed via IncuCyte Imaging System and flow cytometry. The mechanism was explored via western blot and RT-qPCR. ... affecting the migration and invasion of the placental trophoblast cells through STAT3 and Vimentin related pathway; thus, TXNIP might be the potential therapeutic target for repairing the … raw chrysolite ff14WebFlow Cytometry 0.25 µg/10 6 cells See below CyTOF-ready Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Immunocytochemistry 5-15 µg/mL See below Please Note: Optimal dilutions should be determined by each laboratory for each application. simple club angebotWebPhospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) detects endogenous levels of Stat3 only when phosphorylated at Tyr705. This antibody does not cross-react with phospho-EGFR or the … raw chs cht