Flow cytometry protocol for thp-1 cell

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August undefined, 2024

WebWith the same method, the IL-1β in THP-1 cells stimulated with 10 ng/mL of S. enteritidis LPS was 34.8%, whereas after selonsertib administration it was detected at 2.0% using flow cytometry (Table 1 and Figure 2). WebJan 13, 2024 · Cell Staining and Flow Cytometry Analysis. THP-1 derived M0 and iDC were cultivated in Corning® 96 Well TC-Treated Microplates (Life Sciences, Tewksbury, Massachusetts, USA) with a volume of 100 …

Phagocytosis assays with different pH-sensitive fluorescent …

WebFlow Cytometry Reagents. Clinical Diagnostics; ... Single-color flow cytometric analysis of Ig Isotype control staining on THP-1 cells. THP-1 cells were incubated at room … WebPublished protocols using the promonocytic cell line THP-1 have tended to result in cells that closely resemble classically-activated macrophages, differentiated in IFNγ and LPS. … shantel sheds ny

Frontiers A THP-1 Cell Line-Based Exploration of Immune …

WebJan 21, 2024 · Affiliation Flow Cytometry Laboratory, Botucatu Medical School - UNESP, Botucatu, Brazil ⨯ . ... under protocol 2.018.399. THP-1 culture and differentiation. THP-1 cells were cultured in 25 cm 2 sterile … WebMar 11, 2024 · We describe and validate a flow cytometry-based protocol for quantifying G4 levels by using the G4-specific antibody BG4 to label standard cultured cells (Hela and THP-1) as well as primary cells obtained from human blood (peripheral blood mononuclear cells (PBMCs)). We additionally determined changes in G4 levels during the cell cycle in ... WebFeb 3, 2024 · Single-cell preparation from different organs, staining of cells with fluorophore-conjugated antibodies, and analysis of the stained cells using flow … pond black foam

BG-flow, a new flow cytometry tool for G-quadruplex quantification …

A novel flow cytometry-based tool for determining the ... - PubMed

WebApr 4, 2024 · When we compare the level of dye transfer between cells, the CFSE dye in U2OS cells seems to be transferred more to THP-1 cells than the Far Red dye in THP-1 cells being transferred to U2OS cells. To clarify whether the dye transfer is driven by the dyes or cells, we switched the labeling dye and performed another coculture (Fig. 5B). … WebSep 25, 2009 · THP-1 cell surface markers for Flow Cytometry - Need a robust marker for undifferentiated THP-1 cells. I am working with THP-1 cells (ATCC TIB 202), a human monocytic cell line. The majority of people that use this cell line are interested in the differentiated macrophage phenotype, which can be achieved by stimulating THP-1 cells … pond bird watchWebApr 14, 2024 · A similar approach has been used to characterize the adherence and transmigration properties of peripheral blood mononuclear cells on human aortic endothelial cells and brain microvascular endothelial cells . One limitation of the flow cytometry-based assay was the inherent variability in the cells used; for example, the passage number of … pond black cat

"WebMar 18, 2024 · This protocol provides instructions to perform a high-throughput small molecule screen using flow cytometry analysis of THP-1 cells, a human monocytic … " - Flow cytometry protocol for thp-1 cell

Flow cytometry protocol for thp-1 cell

Flow Cytometry Sample Preparation Protocols - Flow Cytometry …

WebJul 14, 2024 · Flow cytometry analysis of treated THP-1 cells was performed for CD68, CD86, and CD206 markers to evaluate M0, M1, and M2 macrophages polarization, respectively. AGS cells were assessed for apoptosis and necrosis by flow cytometry after labeling with Annexin V-FITC and propidium iodide. Web5 Collect 2 million cytotoxic T cells per sample (i.e. per well) from an on-going culture 6 Spin them down at 350 x gfor 00:05:00 at 4 °C and re-suspend them in fresh media at 1 million per mL concentration 7 Supplement T cells with 200 IU/mL rIL2 8 Aspirate the culture media from each of the 24-well-plate wells that contain a sample. Try to aspirate as much as …

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WebFlow Cytometry Blocking Controls Fc receptors are found on monocytes, macrophages, dendritic cells and B cells. ... have been developed which, when added to a staining protocol, can ensure that only antigen specific … Web1 day ago · An increase in exposure of β-(1,3)-glucan and chitin on the cell surface was also observed through flow cytometry. Interestingly, we observed a decrease in the phagocytosis rate when the Δcdc50 mutant was co-incubated with THP-1 macrophages. The Δcdc50 mutant also exhibited weakened virulence in nematode survival tests.

WebJun 5, 2015 · At 24 hpi, IE2-EYFP positive cells were measured by flow cytometry; (b) THP-1 monocytes were differentiated to THP-1 derived macrophages by the addition of PMA for 48 h followed by infection of the obtained differentiated THP-1 cells with TB40E/IE2-EYFP at an MOI of 0.1. At 24 hpi, IE2-positive cells were detected by flow … WebApr 14, 2024 · Cell culture. MOLM13, MV411 and THP-1 cells were obtained from the Sanger Institute cancer cell collection and were grown in 1640-RPMI (Gibco, 21875034) supplemented with 10% FBS (Sigma, F4135 ...

WebJun 18, 2024 · This protocol provides instructions to perform a high-throughput small molecule screen using flow cytometry analysis of THP-1 cells, a human monocytic … WebFeb 21, 2024 · THP-1 cells (2 × 10 5) were seeded and Staphylococcus aureus or Escherichia coli particles labeled with pHrodo red and pHrodo green were added. After 1 …

WebCell cycle assay protocols for flow cytometry. Vybrant DyeCycle Violet Stain. Vybrant DyeCycle Green and Orange Stains. Vybrant DyeCycle Ruby Stain. FxCycle Violet …

WebWith the same method, the IL-1β in THP-1 cells stimulated with 10 ng/mL of S. enteritidis LPS was 34.8%, whereas after selonsertib administration it was detected at 2.0% using … shantel smallWebCell cycle assay protocols for flow cytometry. Vybrant DyeCycle Violet Stain. Vybrant DyeCycle Green and Orange Stains. Vybrant DyeCycle Ruby Stain. FxCycle Violet Stain. FxCycle Violet Ready Flow Reagent. FxCycle PI/RNase Staining Solution. FxCycle Far Red Stain. CellEvent Senescence Green Flow Cytometry Assay Kit. shantel smithWebThe first step to isolating your cells of interest begins with forward scatter (FSC) and side scatter (SSC). Larger, more complex cells will be higher in both parameters. Knowing the size and makeup of your cells of interest is key to gating accurately. If cell lines are being used, the FSC/SSC should show one main population of cells: this ... pond bird netting shantel smith comedianWebAug 8, 2015 · Analysis of CD206 plasma membrane expression by flow cytometry. THP-1 monocytes were seeded in T25 flask at 2.5 × 10 6 cells/T25 and differentiated with PMA. … pond boathouseWebSep 1, 2015 · THP-1, a human monocytic cell line and its derived macrophages serve as a useful cell culture model for mechanistic studies of hCMV infection and its underlying biology. A major methodological challenge is the lack of a quick and reliable tool to accurately determine the efficiency of hCMV infection in THP-1 derived macrophages. shantel smith survivor twitterWebJun 26, 2024 · We found that the baseline median fluorescent intensity (MFI) of THP-1 cells changed depending on the time of sample acquisition to the flow cytometer. To solve this problem, a fixation step was introduced in different stages of the assay's protocol, obtaining more reproducible and sensitive results when a post-LC3 staining fixation was ... shantel smith pastor washington dc